全文获取类型
收费全文 | 446942篇 |
免费 | 55478篇 |
国内免费 | 1954篇 |
出版年
2018年 | 4742篇 |
2017年 | 4300篇 |
2016年 | 5915篇 |
2015年 | 8558篇 |
2014年 | 9902篇 |
2013年 | 13488篇 |
2012年 | 15170篇 |
2011年 | 15186篇 |
2010年 | 10107篇 |
2009年 | 9236篇 |
2008年 | 12675篇 |
2007年 | 12895篇 |
2006年 | 11926篇 |
2005年 | 11341篇 |
2004年 | 10868篇 |
2003年 | 10712篇 |
2002年 | 10209篇 |
2001年 | 20236篇 |
2000年 | 20449篇 |
1999年 | 16175篇 |
1998年 | 5679篇 |
1997年 | 6180篇 |
1996年 | 5890篇 |
1995年 | 5658篇 |
1994年 | 5554篇 |
1993年 | 5565篇 |
1992年 | 13619篇 |
1991年 | 13410篇 |
1990年 | 12812篇 |
1989年 | 12569篇 |
1988年 | 11425篇 |
1987年 | 11071篇 |
1986年 | 10333篇 |
1985年 | 10268篇 |
1984年 | 8606篇 |
1983年 | 7409篇 |
1982年 | 5709篇 |
1981年 | 5068篇 |
1980年 | 4872篇 |
1979年 | 8056篇 |
1978年 | 6438篇 |
1977年 | 5862篇 |
1976年 | 5511篇 |
1975年 | 6074篇 |
1974年 | 6255篇 |
1973年 | 6189篇 |
1972年 | 5585篇 |
1971年 | 5136篇 |
1970年 | 4280篇 |
1969年 | 4124篇 |
排序方式: 共有10000条查询结果,搜索用时 17 毫秒
11.
The Consequences of Precipitation Seasonality for Mediterranean-Ecosystem Vegetation of South Africa
Globally, mediterranean-climate ecosystem vegetation has converged on an evergreen, sclerophyllous and shrubby growth form. The particular aspects of mediterranean-climate regions that contribute to this convergence include summer droughts and relatively nutrient-poor soils. We hypothesised that winter-precipitation implies stressful summer droughts and leaches soils due to greater water availability (i.e. balance between precipitation and potential evapotranspiration; P–PET) during cold periods. We conducted a comparative analysis of normalised difference vegetation indices (NDVI) and edaphic and climate properties across the biomes of South Africa. NDVI was strongly correlated with both precipitation and P–PET (r2 = 0.8). There was no evidence, however, that winter-precipitation reduces NDVI in comparison to similar amounts of summer-precipitation. Base saturation (BS), a measure of soil leaching was, however, negatively related to P–PET (r2 = 0.64). This led to an interaction between P–PET and BS in determining NDVI, indicating the existence of a trade-off between water availability and soil nutrients that enables NDVI to increase with precipitation, despite negative consequences for soil nutrient availability. The mechanism of this trade-off is suggested to be that water increases nutrient accessibility. This implies that along with nutrient-depauperate geologies and long periods of time since glaciation, the winter-precipitation may have contributed to the highly leached status of the soils. Since many of the ecophysiological characteristics of mediterranean-ecosystem flora are associated with low nutrient availabilities (e.g. evergreen foliage, sclerophylly, cluster roots), we conclude that mediterranean-climates promote convergence of growth-forms in these regions through high leaching capacity. 相似文献
12.
A highly abundant satellite DNA comprising 17% of the Tribolium castaneum
(Insecta, Coleoptera) genome was cloned and sequenced. The satellite
monomer is 360 bp long, has a high A+T content of 73%, and lacks
significant internal substructures. The sequence variability is 3.6%,
essentially due to random distribution of single-point mutations. The
satellite is evenly distributed in the regions of centromeric
heterochromatin of all 20 chromosomes, as shown by fluorescent in situ
hybridization. Comparison of T. castaneum satellite with those from three
different but congeneric species reveals the highest sequence similarity of
47.1% with the satellite from the sibling species Tribolium freemani. The
phylogenetic relationships among Tribolium species deduced from satellite
sequence agree with those based on karyological, chemotaxonomic, and
hybridization data. This indicates a parallel in the divergence of
satellites and some genetic and cytogenetic characters. Despite low mutual
sequence similarity, which makes them species-specific, Tribolium
satellites have a common structural characteristic: a block of about 95%
A+T content, 20 to 42 bp long, flanked at one side by an inverted repeat
which can potentially form a thermodynamically stable dyad structure. Since
similar structural features are found in centromeric DNA of Saccharomyces
cerevisiae and Chironomus pallidivittatus, their possible importance in
centromere function may be inferred.
相似文献
13.
14.
15.
16.
17.
E. Smyk-Randall O. R. Brown A. Wilke A. Eisenstark D. H. Flint 《Free radical biology & medicine》1993,14(6):609-613
The effects of near ultraviolet (NUV) light on a NUV chromophore-containing oxidant-sensitive enzyme, dihydroxyacid dehydratase (DHAD), were measured in seven strains of Escherichia coli. The strains differed in production of the oxidant-defense enzymes, superoxide dismutases (Fe-SOD and Mn-SOD), and catalases HPI and HPII. With the stress of aerobic growth but without NUV exposure, the strains lacking either Fe or Mn SOD or both SODs had 57%, 25%, and 12%, respectively, of the DHAD-specific activity of the parent (K12) strain. Under the same conditions, the catalase strains that were wild type, overproducing, and deficient had comparable DHAD-specific activities. When aerobic cultures were exposed for 30 min to NUV with a fluence of 216 J/m2/s at 310–400 nm, the percentage decreases in DHAD-specific activities were similar (ranging from 75% to 89%) in strains with none, either, or both SODs missing, and in the catalase-overproducing strain. However, the decreases were only 58% and 52% in the strain with catalase missing and in its parent, respectively. The NUV-induced loss of DHAD enzyme activity was not accompanied by any detectable loss of the DHAD protein as measured by polyclonal antibody to DHAD. 相似文献
18.
The ADP/ATP carrier is the 32-kilodalton receptor for an NH2-terminally myristylated src peptide but not for pp60src polypeptide. 总被引:2,自引:0,他引:2 下载免费PDF全文
Membrane binding of pp60src is initiated via its myristylated NH2 terminus. To identify a candidate pp60src docking protein or receptor in the membrane, a radiolabelled peptide corresponding to the pp60src NH2-terminal membrane binding domain was cross-linked to fibroblast membranes and found to specifically label a 32-kDa protein. This protein was purified by appending an affinity tag to the peptide probe so that the cross-linked complex could be isolated via affinity chromatography. Microsequencing indicated that the 32-kDa protein was the mitochondrial ADP/ATP carrier (AAC). This result was further confirmed by the ability of an antibody to the AAC to immunoprecipitate the cross-linked complex, by the ability of certain inhibitors of the AAC to block cross-linking, and by membrane fractionation to show that complex formation occurred essentially exclusively in the mitochondrial fraction. While the AAC bound the myristyl-src peptide in a specific manner both in vitro and in vivo, its localization to the inner membrane of the mitochondrion precludes its being a pp60src binding protein. An analysis of pp60v-src binding in vitro was consistent with this expectation. Thus, use of a myristyl-src peptide revealed an unexpected and previously unidentified binding capacity of the AAC, most likely related to the ability of long-chain fatty acyl coenzyme As to serve as AAC inhibitors. The amphipathic nature of the pp60src NH2 terminus suggests alternative strategies for uncovering pp60src membrane binding species. 相似文献
19.
20.